Analysis Note

ControlPOSITIVE CONTROL: Activated microglial cells.

Application

Anti-PECAM-1 Antibody, clone TLD-3A12, azide free detects level of PECAM-1 & has been published & validated for use in ELISA, FC, WB, IH.

Research CategoryCell Structure

Research Sub CategoryAdhesion (CAMs)

Flow Cytometry: 1:50-1:100

Immunohistology: frozen sections (-20°C methanol fixation), not recommended for traditional formalin fixed tissues.

ELISA

Western Blotting: 1:500. MW = 130-140 kDa

Optimal working dilutions must be determined by end user.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

Activated, Lewis rat derived microglial cells.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Format: Purified

Purified immunoglobulin by affinity chromatography on Protein G. Liquid in phosphate buffered saline containing no preservatives.

Specificity

Rat PECAM-1. Antibody gives positive reaction at high dilutions against recombinant rat PECAM-1, confirming specificity.

Storage and Stability

Maintain at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.